Identification of Triton X-100 Insoluble Membrane Domains in the Yeast Saccharomyces cerevisiae
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چکیده
منابع مشابه
The Triton X-100 and High Salt Resistant Residue of Saccharomyces cerevisiae Nuclear Membranes, II
Nuclear Membrane, Insoluble Membrane Residue, G 1 Phase of Cell Cycle, a Mating Pheromone, Anti-Lamine Antibodies The Triton X-100 and High salt resistant residue of the nuclear envelope of Saccharomyces cerevisiae cells arrested in the G 1 phase of cell cycle with a mating pheromone was isolated. It showed the same electrophoretic protein pattern as the insoluble residue isolated from log-phas...
متن کاملThe Triton X-100 and high salt resistant residue of Saccharomyces cerevisiae nuclear membranes.
Saccharomyces cerevisiae nuclear membranes were prepared from isolated nuclei by digesting chromatin with deoxyribonuclease after an initial treatment of nuclei with very diluted buffers. When the nuclear membranes were treated with 5% Triton X-100 and 1M NaCl an insoluble fibrous net was obtained which consisted mainly of protein with Mr values of 85 000, 48 000, 45 000, 39 000 and 31 000. Lam...
متن کاملCD44 exhibits a cell type dependent interaction with triton X-100 insoluble, lipid rich, plasma membrane domains.
CD44 is an abundant, widely expressed transmembrane glycoprotein which can act as a receptor for the extracellular matrix glycosaminoglycan, hyaluronan. Biochemical and morphological studies have demonstrated that in fibroblasts a significant of the CD44 population is resistant to Triton X-100 extraction and that the detergent insoluble protein is co-localized with components of the cortical cy...
متن کاملCharacterization of Encapsulated Berberine in Yeast Cells of Saccharomyces cerevisiae
Berberine was loaded in yeast cells of Saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. The yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. In this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order...
متن کاملCharacterization of Encapsulated Berberine in Yeast Cells of Saccharomyces cerevisiae
Berberine was loaded in yeast cells of Saccharomyces cerevisiaeas a novel pharmaceutical carrier to improve the treatment ofmany diseases. The yeast-encapsulated active materialsshowedhigh stability and bioavailability due to the enhanced solubility and sustained releasing. In this study, different characteristics of prepared berberine loaded yeast cells (loading capacity, release kinetic order...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.51.32975